1. Cancer Gene Discovery: Past and Present Christopher R. Clark, Wilaiwan DuRose, and Timothy K. Starr Part I: Cancer Gene discovery In Silico based on Sequencing Data 2. Identification of Cancer Driver Genes from a Custom Set of Next Generation Sequencing Data Shu-Hsuan Liu and Wei-Chung Cheng 3. Cancer Gene Discovery by Network Analysis of Somatic Mutations using the MUFFINN Server Heonjong Han, Ben Lehner, and Insuk Lee 4. Identifying Driver Interfaces Enriched for Somatic Missense Mutations in Tumors Kivilcim Ozturk and Hannah Carter 5. Identification of Cancer Genes Based on de novo Transposon Insertion Site Analysis using RNA and DNA Sequencing Aaron Sarver PartII: Cancer Gene Discovery using Cell and Organoid Systems 6. A Cell-based Method for Identification of Chemotherapy Resistance Cancer Genes Raffaele Hellweg, Ashley Mooneyham, and Martina Bazzaro 7. Engineering a Bioartificial Human Colon Model through Decellularization and Recellularization Huanhuan Joyce Chen and Michael L. Shuler 8. Mutagenesis Screens for Prostate Cancer using Replication-Incompetent Lentiviral Vectors Grant D. Trobridge 9. Arrayed shRNA Screening to Identify Suppressors of Anchorage-independent Growth Ugur Eskiocak 10. Genome-wide CRISPR/Cas9 Screening for Identification of Cancer Genes in Cell Lines Charles H. Adelmann, Tim Wang, David M. Sabatini, and Eric S. Lander 11. CRISPR/Cas9 Based Positive Screens for Cancer Related Traits Nicholas J. Slipek, Jyotika Varshney, and David A. Largaespada 12. Ex vivo Transposon-Mediated Genetic Screens for Cancer Gene Discovery Kathryn A. O'Donnell, Yabin Guo, Shruthy Suresh, Barrett L. Updegraff, and Xiaorong Zhou Part III: Cancer Gene Discovery using Animal Models 13. Cancer Gene Discovery Utilizing Sleeping Beauty Transposon Mutagenesis Kelsie L. Becklin, Branden A. Smeester, and Branden S. Moriarity 14. PiggyBac Transposon-based Insertional Mutagenesis in Mice Mathias J. Friedrich, Iraad F. Bronner, Pentao Liu, Allan Bradley, and Roland Rad 15. Liver-specific Delivery of Sleeping Beauty Transposon System by Hydrodynamic Injection for Cancer Gene Validation Amy P. Chiu and Vincent W. Keng 16. Engineering Large Genomic Rearrangement in Mouse Embryonic Stem Cell for Cancer Gene Discovery Yuen-Yi Tseng and Anindya Bagchi sakriti opis