This book is of counterbalance immediate sequential effect crossover design. the book is mainly written for bring up a new knowledge and new method of constructing counterbalance immediate sequential effect. The book is divided into five chapters, chapter one is on introduction that talks about principles of experimental, Latin squares, crossover designs; chapter two describes counterbalancing for immediate sequential effects. Chapter three gives method of construction for incomplete counterbalancing for immediate sequential and four explained subject size counterbalancing for immediate sequential effects in which number of period is less or equal to treatment number and and the last chapter talk about period size counterbalancing for immediate sequential effects. sakriti opis