Foreword. Serge Delrot\n1. Grapevine roots and soil environment: growth, distribution and function. Felicidad de Herralde, Robert Savé, Xavier Aranda, Carme Biel, M Mar\n2. Radiation balance in vineyards. Pilar Baeza, Patricia Sánchez-De-Miguel, José Ramón Lissarrague\n3. Vegetative growth, total leaf area and surface indexes. Patricia Sánchez de Miguel, Pilar Baeza; Pedro Junquera, José Ramón Lissarrague\n4. Vegetative growth, reproductive development and vineyard balance. L.G. Santesteban, C. Miranda, J.B. Royo\n5. Methodologies for the measurement of water flow in grapevines. J.M. Escalona, M. Ribas-Carbó\n6. Methods for assessment of hydraulic conductance and embolism extent in grapevine organs. Claudio Lovisolo, Sara Tramontini\n7. Comparison of three operational tools for the assessment of vine water status : stem water potential, carbon isotope discrimination measured on grape sugar and water balance. Cornelis van Leeuwen, Philippe Pieri and Philippe Vivin\n8. Gas-exchange and chlorophyll fluorescence measurements in grapevine leaves in the field. Alexander Gallé, Jaume Flexas\n9. Measuring Water Use Efficiency in Grapevines. Hipolito Medrano, Jaume Flexas, M Ribas Carbó, J. Gulias\n10. Use of thermal imaging in viticulture: current application and future prospects. J. Miguel Costa, O.M. Grant, Manuela.M. Chaves\n11. Grapevine fruiting cuttings: an experimental system to study grapevine physiology under water deficit conditions. M. Carmen Antolin, Hector Santesteban, Marouen Ayari, Jone Aguirreolea, Manuel Sanchez-Diaz\n12. Nutritional deficiencies. Luigi Bavaresco, Matteo Gatti, Mario Fregoni\n13. Polyamines in grapevine research. Panagiotis N. Moschou, Kalliopi A. Roubelakis-Angelakis\n14. Field assessment and diagnostic methods for detection of grapevine viruses. Giorgio Gambino, Elisa Angelini, Ivana Gribaudo\n15. Real-time PCR detection methods for economically important grapevine related bacteria. Matjaz Hren, Tanja Dreo, Jana Skubic, Petra Nikoli?, Kristina Gruden, Marina Dermastia, Maja Ravnikar\n16. Field assesment and diagnostic methods for detection of grapevine phytoplasmas. Elisa Angelini\n17. NITC, new tools to control phytochemical treatments and traceability. Vincent de Rudnicki\n18. Isolation and use of protoplasts from grapevine tissues. Natacha Fontes, Hernâni Gerós, Anastasia K. Papadakis, Serge Delrot, Kalliopi A. Roubelakis-Angelakis\n19. RNA extraction from grapevine woody canes for gene expression analysis by real-time RT-PCR. Sophie Bordiec, Fanja Rabenoelina, Florence Mazeyrat-Gourbeyre, Christophe Clément, Fabienne Baillieul\n20. A Method for Isolating Total RNA from Mature Buds and other woody tissues of Vitis vinifera. Etti Or\n21. RNA extraction from young, acidic berries and other organs from Vitis vinifera L. Charles Romieu\n22. Transcriptomics: methods for microarray data analysis and visualization using the Affymetrix GeneChip® Vitis vinifera genome array. Karen A. Schlauch, Jerôme Grimplet, John Cushman, Grant R. Cramer\n23. Visualisation of transcriptomics data in metabolic pathways. Ana Rotter, Matjas Hren, Bjorn Usadel, Kristina Gruden\n24. Small RNA extraction and expression analysis by Northern blot. Giorgia Batelli, Giorgio Gambino, Erica Mica, Andrea Schubert, Andrea Carra\n25. A Method for Isolating Total Proteins from Mature Buds and other woody tissues of Vitis vinifera. Etti Or\n26. Expression analysis in grapevine by in situ hybridization and immunohistochemistry. Steven Colas, Lucile Jacquens, Sébastien Manteau, Jérome Devy, Geneviève Conéjéro, Christophe Clément, Fabienne Baillieul, Florence Mazeyrat-Gourbeyre, Laurence Monti-Dedieu\n27. Marker development for important grapevine traits by genetic diversity studies and investigation of differential gene expression. Achim Schmitt, Martina Rex, Stefan Ebert, Wolfgang Friedt, Reinhard Töpfer, Eva Zyprian\n28. Phenolic maturity in red grapes. Montse N