Part I: Generating Hypothesis

1. Double Immunohistochemistry and Digital Image Analysis

   Pablo Moreno-Ruiz, Lina Wik Leiss, Artur Mezheyeuski, and Monika Ehnman

2. Multispectral Fluorescence Imaging Allows for Distinctive Topographic Assessment and Sub-Classification of Tumor Infiltrating and Surrounding Immune Cells

   Claudia Wickenhauser, Daniel Bethmann, Zipei Feng, Shawn M. Jensen, Carmen Ballesteros-Merino, Chiara Massa, Andre Steven, Marcus Bauer, Peter Kaatzsch, Nikolaos Pazaitis, Georgiana Toma, Carlo B. Bifulco, Bernard A. Fox, and Barbara Seliger

3. Systems Level Immune Monitoring by Mass Cytometry

   Tadepally Lakshmikanth and Petter Brodin

4. Immune Monitoring of Cancer Patients by Multi-Color Flow Cytometry

   Neo Shiyong, Aine O'Reilly, and Yago Pico de Coaña

5. High-Throughput, Fast and Sensitive Immunopeptidomics Sample Processing for Mass Spectrometry

   Fabio Marino, Chloe Chong, Justine Michaux, and Michal Bassani-Sternberg

6. Identification of Immune Modulatory miRNAs by miRNA Enrichment via RNA Affinity Purification

   Uta Sandy Tretbar, Michael Friedrich, Maria-Filothei Lazaridou, and Barbara Seliger

Part II: Functional Assays

1. Expansion of Tumor-Infiltrating Lymphocytes from Melanoma Tumors

   Stina Wickström and Tanja Lövgren

2. Establishment of Bi-Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies

   Nataly Cruz Rodríguez, Johanna Lineros, Carol Stefany Rodríguez, Lina María Martínez, and Josefa Antonia Rodríguez

3. Assessment of Anti-Tumor T Cell Responses by Flow Cytometry after Co-Culture of Tumor Cells with Autologous Tumor-Infiltrating Lymphocytes

   Jeroen Melief, Stina Wickström, Rolf Kiessling, and Yago Pico de Coaña

4. Expansion and Determination of Antigen-Reactive T Cells by Flow Cytometry

Alexander Martens, Graham Pawelec, and Christopher Shipp

1. Assessing Canonical and Adaptive Natural Killer Cell Function in Suppression Assays In Vitro

Dhifaf Sarhan and Jeffrey S. Miller

1. Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Chromium Release Assay

Irene van der Haar Avila, Patricia Marmol, Rolf Kiessling, and Yago Pico de Coaña

1. Evaluating Antibody-Dependent Cell-Mediated Cytotoxicity by Flow Cytometry

Irene van der Haar Avila, Patricia Marmol, Jeannette Cany, Rolf Kiessling, and Yago Pico de Coaña

Part III: Animal Models

1. Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models

Kristina Witt and Andreas Lundqvist

1. Establishment of Melanoma Tumor Xenograft Using Single Cell Line Suspension and Co-Injection of Patient-Derived T Cells in Immune Deficient NSG Mice

Fríða Björk Gunnarsdóttir, Rolf Kiessling, and Yago Pico de Coaña

1. Analysis of Tissue Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry

Felipe Galvez-Cancino, Ernesto Lopez, and Alvaro Lladser