Part I: An Overview

1. Cell Free DNA: Applications in Different Diseases

Rossella Ranucci

1. Cell Free DNA: An Overview of Sample Types and Isolation Procedures Filippo Martignano

Part II: A Liquid Biopsy for Cancer Disease

1. Circulating Cell Free DNA and Cancer Therapy Monitoring: Methods and Potential Peter B. Gahan

2. Cell Free DNA in the Liquid Biopsy Context: Role and Differences between ctDNA and CTC Marker in Cancer Management

Pietro Fici

Part III: Methods in Cancer 5. Cell Free DNA Integrity: Applications

Sara Ravaioli

1. Copy Number Variation Analysis on Cell Free Serum DNA

Heidi Schwarzenbach

1. Studying Copy Number Variations in Cell Free DNA: The Example of AR in Prostate Cancer

Samanta Salvi and Valentina Casadio

1. dPCR Mutational Analyses in Cell-free DNA: A Comparison With Tissues

Takashi Takeshita and Hirotaka Iwase

1. From cfDNA to Sequencing: Workflows and Potentials

Michela Tebaldi and Samanta Salvi

Part IV: Epigenetic Modifications

1. Epigenetic Characterization of Cell Free DNA Giorgia Gurioli

2. Quantitative Methylation-specific PCR: A Simple Method for Studying Epigenetic Modifications of Cell Free DNA

Luca Sigalotti, Alessia Covre, Francesca Colizzi, and Elisabetta Fratta

Part V: Fetal and Pediatric Diseases

1. Cell Free DNA in Pediatric Rhabdomyosarcoma: Potential and Challenges

Lucia Tombolan, Angelica Zin, and Gianni Bisogno

1. Bioinformatics Pipeline for Accurate Quantification of Fetal DNA Fraction in Maternal Plasma

Meng Ni, Xianlu Laura Peng, and Peiyong Jiang

Part VI: Physical Activity

1. Circulating Cell-Free DNA in Physical Activities

LN Vittori, A. Tarozzi, and P. Maietta Latessa

Part VII: Urinary Cell Free DNA

1. Urinary Cell Free DNA: Potential and Applications Samanta Salvi and Valentina Casadio

2. Urinary Cell Free DNA: Isolation, Quantification, Quality Assessment

Valentina Casadio and Samanta Salvi