Introduction: When should I use my UHPLC as a UHPLC? PART 1: HARDWARE AND SOFTWARE, SEPARATION MODES, MATERIALS Modern HPLC/UHPLC equipment Requirements for the different parts of HPLC/UHPLC equipment The column oven - a simple matter? The problem of the extra column dispersion in an HPLC/UHPLC equipment The gradient: Requirements, optimal operation, tricks and pitfalls Hardware requirements for LC coupling with different mass spectrometers 2D-Chromatography - potentials and limitations Selecting the right column materials in HPLC and UHPLC Qality by Design in HPLC Method Development PART 2: USE CASES AND TRENDS What should the software be able to do, so that the hardware can be used to its full potential? Aspects of modern HPLC - a user s experience report Hints and recommendations for optimal use of HPLC equipment Using UHPLC to solve the compound separation problem Equipment review (Agilent, Shimadzu, ThermoScientific) sakriti opis