Part I: Properties, Design, Synthesis and Characterization of AMPs 1. Antimicrobial Peptides: An Introduction Evan F. Haney, Sarah C. Mansour, and Robert E.W. Hancock 2. Tools for Designing Amphipathic Helical Antimicrobial Peptides Davor Juretic, Damir Vukicevic, and Alessandro Tossi 3. Chemical Synthesis of Antimicrobial Peptides Lena Münzker, Alberto Oddo and Paul R. Hansen 4. Microwave-Assisted Synthesis of Antimicrobial Peptides Suhas Ramesh, Beatriz G. de la Torre, Fernando Albericio, Hendrik G. Kruger, and Thavendran Govender 5. High Performance Liquid Chromatography and Mass Spectrometry-Based Design of Protolytically Stable Antimicrobial Peptides Mojtaba Bagheri and Robert E. W. Hancock 6. Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR Reinhard Wimmer and Lars Erik Uggerhøj 7. Mass Spectrometry Approaches for Determining the Structure of Antimicrobial Peptides Jiongyu Liu and Jianping Jiang Part II: Studying Interactions of AMPs with Model Membranes and Bacteria 8. Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptides-Lipid Bilayers Interactions Shima Arasteh and Mojtaba Bagheri 9. Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides Mauricio Arias, Elmar J. Prenner and Hans J. Vogel 10. Fluorescence and Absorbance Spectroscopy Methods to Study Membrane Perturbations by Antimicrobial Host Defense Peptides Mauricio Arias and Hans J. Vogel 11. Applying Fluorescence Correlation Spectroscopy to Investigate Peptide-Induced Membrane Disruption Kasper Kristensen, Jonas R. Henriksen, and Thomas L. Andresen 12. Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action Bimal Jana, Kristin Renee Baker, and Luca Guardabassi 13. Using Confocal Microscopy and Computational Modeling to Investigate the Cell Penetrating Properties of Antimicrobial Peptides Gabriel Del Rio, Edda Klipp, and Andreas Herrmann 14. Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA Peter Fojan and Leonid Gurevich 15. Protocols for studying the interaction of MSI-78 with the Membranes of Whole Gram-positive and Gram-negative Bacteria by NMR Nury P. Santisteban , M. R. Morrow, and V. Booth 16. Preparation of membrane models of Gram-negative bacteria and their interaction with Antimicrobial Peptides studied by CD and NMR Rickey P. Hicks 17. Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells using Circular Dichroism Concetta Avitabile, Luca Domenico D'Andrea and Alessandra Romanelli 18. Studying the Mechanism of Membrane Permeabilization induced by Antimicrobial Peptides using Patch-clamp Techniques Giorgio Rispoli 19. Assays for Identifying Inducers of the Antimicrobial Peptide LL-37 Frank Nylen, Peter Bergman, Gudmundur H. Gudmundsson, and Birgitta Agerberth 20. Methods for Elucidating the Mechanism of Action of Proline-rich and other Non-lytic Antimicrobial Peptides Monica Benincasa, Giulia Runti, Mario Mardirossian, Renato Gennaro,and Marco Scocchi 21. The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus Aureus Investigated by ATP-leakage, DNA-binding Analysis and the Expression of a LexA Controlled Gene, recA Sanne Gottschalk and Line Elnif Thomsen Part III: Assaying Selected Biological Activities of AMPs 22. Methods for Investigating Biofilm Inhibition and Degradation by Antimicrobial Peptides Li-av Segev-Zarko and Yechiel Shai 23. Protocols for Studying Inhibition and Eradication of Bacterial Biofilms by Antimicrobial Peptides Vijayalekshmi Sarojini 24. Protocols for Studying Antimicrobial Peptides (AMPs) as Anti-cancer Agents Laurence Madera and David W. Hoskin 25. Using Oral a sakriti opis