PART I: Ligand Binding Diffusion Interaction between macromolecules and ligands Identical independent binding sites Non-identical independent binding sites Identical interacting binding sites, cooperativity Non-identical interacting binding sites PART II: Enzyme Kinetics Reaction Order Steady-state kinetics and the Michaelis-Menten Equation Reversible enzyme reactions Enzyme inhibition Multi-substrate reactions Rate equations of complex enzyme mechanisms Allosteric enzymes pH and temperature dependence Isotope exchange kinetics and isotope effects Ribozymes Enyzmes with polymeric substrates Immobilized enzymes Kinetics of transport processes Enzyme reactions at membrane interfaces PART III: Methods Methods for investigating multiple equilibria Electrochemical methods Calorimetry Spectroscopic methods Measurement of fast reactions sakriti opis