1 - DNA synthesis and genome engineering 1.1 Competition and the Future of Reading and Writing DNA 1.2 Trackable multiplex recombineering (TRMR) and next-generation genome design technologies 1.3 Site-directed genome alteration with engineered recombinases/ZFs 1.4 Rational efforts to streamline the E. coli genome 1.5 Functional requirements in the program and the cell chassis for next generation synthetic biology 2 - Parts and devices supporting control of protein expression and activity 2.1 Constitutive and regulated promoter engineering 2.2 Splicing and Alternative Splicing Impact on Gene Design 2.3 Application of RNA interference 2.4 RNA switches 2.5 Programming Gene Expression by Engineering Transcript Stability Control and Processing in Bacteria 2.6 Standardized functional peptides and their application in superfunctionalizing proteins 3 - Parts and devices supporting spatial engineering 3.1 Metabolic channeling using DNA as a scaffold 3.2 Synthetic RNA scaffolds for spatial engineering in cells 3.3 Sequestered: Design and Construction of Synthetic Organelles 4 - Early applications of synthetic biology: pathways, therapies, and cell-free synthesis 4.1 Cell free protein synthesis: an emerging technology for understanding, harnessing, and expanding the capabilities of biological systems 4.2 Applying Advanced DNA Assembly Methods to Generate Pathway Libraries 4.3 Synthetic Biology in Immunotherapy 05 - Societal ramifications of synthetic biology Synthetic Biology: From Genetic Engineering 2.0 to Responsible Research and Innovation