Section 1. Basics of EUS 1. Principles of Ultrasound 2. Equipment 3. Training and Simulators 4. Indications, Preparation, and Adverse Effects 5. New Techniques in EUS: Real-Time Elastography, Contrast-Enhanced EUS, and Fusion Imaging Section 2. Mediastinum 6. How to Perform EUS in the Esophagus and Mediastinum 7. EUS(B) and EBUS in Non-Small-Cell Lung Cancer 8. EUS in Esophageal and Gastric Cancer 9. EUS in the Evaluation of Posterior Mediastinal Lesions Section 3. Stomach 10. How to Perform EUS in the Stomach 11. Subepithelial Lesions Section 4. Pancreas and Biliary Tree 12. How to Perform EUS in the Pancreas, Bile Duct, and Liver 13. EUS in Inflammatory Disease of the Pancreas 14. EUS and Pancreatic Tumors 15. EUS in the Evaluation Pancreatic Cysts 16. EUS in Bile Duct, Gallbladder, and Ampullary Lesions Section 5. Anorectum 17. How to Perform Anorectal EUS 18. EUS in Rectal Cancer 19. Evaluation of the Anal Sphincter by Anal EUS Section 6. EUS-Guided Tissue Acquisition 20. How to Perform EUS-Guided Fine-Needle Aspiration 21. Techniques for EUS-Guided Fine-Needle Biopsy 22. Cytology Primer for Endosonographers 23. EUS-Guided Drainage of Pancreatic Fluid Collections 24. EUS-Guided Drainage of the Biliary-Pancreatic Ductal Systems and Gallbladder 25. EUS-Guided Ablation Therapy and Celiac Plexus Interventions 26. EUS-Guided Anastomosis, Drainage of Abdominal-Pelvic Fluid Collections, and Vascular Interventions sakriti opis