1. Fabrication of Glass Microfluidic Devices Christopher T. Culbertson, Jay Sibbitts, Kathleen Sellens, and Shu Jia 2. Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices Ashis Kumar Sen, Abhishek Raj, Utsab Banerjee, and Sk Rameez Iqbal 3. Sample Injection Techniques James M. Karlinsey 4. Sample Preconcentration Protocols in Microfluidic Electrophoresis Fumihiko Kitagawa and Koji Otsuka 5. Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection Lucas Paines Bressan, Dosil Pereira de Jesus, Dulan Bandara Gunasekara, Susan Marie Lunte, and José Alberto Fracassi da Silva 6. Micellar Electrokinetic Chromatography Braden C. Giordano, Ronald Seifert, and Greg E. Collins 7. Microchip Isotachophoresis: Analysis of Pharmaceuticals Marián Masár and Jasna Hradski 8. Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination Stefan Nagl 9. Nanochannel Gradient Separations Michael A. Startsev and David W. Inglis 10. Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications Cyro L.S. Chagas, Thiago M.G. Cardoso, and Wendell K.T. Coltro 11. Band Broadening Theories in Capillary Electrophoresis Sandip Ghosal 12. Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation Debashis Dutta 13. Microchip Electrophoresis Tools for the Analysis of Small Molecules Federico J.V. Gomez and María Fernanda Silva 14. Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS): Swab Sample-In to DNA Profile-Out Jianing Yang, Cedric Hurth, Alan Nordquist, Stan Smith, and Frederic Zenhausern 15. Achieving Stable Electrospray Ionization Mass Spectrometry (ESI-MS) Detection from Microfluidic Chips Iulia M. Lazar 16. Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow Ling Xia and Debashis Dutta sakriti opis