Part I: Introduction

1. Transgenic Cotton: From Biotransformation Methods to Agricultural Application

Baohong Zhang

Part II: Transformation

1. Agrobacterium -mediated Genetic Transformation of Cotton

Baohong Zhang

1. Biolistic Transformation of Cotton Zygotic Embryo Meristem

K. Rajasekaran

1. Novel Pollen Magnetofection System for Transformation of Cotton Plant with Magnetic Nanoparticles as Gene Carriers

Rui Zhang, Zhigang Meng , Muhammad Ali Abid, and Xiang Zhao

1. Biolistic Transformation of Cotton Embryogenic Cell Suspension Cultures

K. Rajasekaran

1. Pollen Tube Pathway-Mediated Cotton Transformation

Min Wang, Runrun Sun, Baohong Zhang, and Qinglian Wang

1. Embryogenic Calli Explants and Silicon Carbide Whisker-mediated Transformation of Cotton ( Gossypium hirsutum L.)

Muhammad Arshad and Shaheen Asad

Part III: Genome Editing and Vigs

1. Genome Editing in Cotton Using CRISPR/Cas9 System

Chao Li and Baohong Zhang

1. Tobacco Rattle Virus Induced Gene Silencing in Cotton

Caiping Cai, Xinyu Wang, Baohong Zhang, and Wangzhen Guo

Part IV: Detection

1. Investigating Transgene Integration and Organization in Cotton ( Gossypium hirsutum L.) Genome

Jun Zhang and Yan Hong

1. Estimating the Copy Number of Transgenes in Transformed Cotton by Real-time Quantitative PCR

Chengxin Yi and Yan Hong

1. Development of an Enzyme-linked Immunosorbant (ELISA) Assay for the Detection of GM Protein in GM Crops/Produce

Suchitra Kamle, Dawei Li, Abhishek Ojha, and Arvind Kumar

1. Screening of Transgenic Cotton Based on a Porous Silicon Biosensor

Liangliang Chen, Peng Li, Xiaoyi Lv, and Ji Ma

1. YC3.60-based Imaging Analysis on Calcium Level in Cotton Cells

Mi Zhang, Jianyan Zeng, Huizhen Cao, and Yan Pei

1. A Simple and Rapid Method for Determining Transgenic Cotton Plants Using a Marker Gene

Baohong Zhang, Hongmei Wang, Fang Liu, and Qinglian Wang

1. A Grafting Technique for Efficiently Transplanting Transgenic Regenerated Plants of Cotton

Min Wang, Qinglian Wang, and Baohong Zhang

1. Inheritance of Transgenes in Transgenic Bt lines Resistance to Helicoerpa armigera in Upland Cotton

Baolong Zhang, Wangzhen Guo, and Tianzhen Zhan

Part V: Application

1. Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics

Hee Jin Kim

1. Overexpression of miRNA in Cotton via Agrobacterium -mediated Transformation

Min Wang, Runrun Sun, Qinglian Wang, and Baohong Zhang

1. Development of Transgenic CryIA(c) + GNA Cotton Plants via Pollen Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover

Zhi Liu, Zhen Zhu, and Tianzhen Zhang

1. Next Generation Transgenic Cotton: Pyramiding RNAi with Bt Counters Insect Resistance

Wei Ma and Tianzhen Zhang

1. Genetic Transformation of Cotton with the Harpin-encoding Gene hpa Xoo of Xanthomonas oryzae pv. Oryzae and Evaluation of Resistance against Verticillium Wilt

Weiguo Miao and Jingsheng Wang

1. Development of Insect-resistant Transgenic Cotton with Chimeric TVip3A Accumulating in

Chloroplasts

Jiahe Wu and Yingchuan Tian

1. Development of Virus Resistance Transgenic Cotton using Cotton Leaf Curl Virus Antisense ßC1 Gene

S.S. Sohrab

Part VI: Risk Assessment

1. Determining Pollen-mediated Gene Flow in Transgenic Cotton

Xiaoping Pan