1. In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging Olivia Farrelly, Paola Kuri, and Panteleimon Rompolas 2. Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells Daniel Haensel, Melissa A. McNeil, and Xing Dai 3. Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction Frédéric Auvré, Julien Coutier, Michèle T. Martin, and Nicolas O. Fortunel 4. Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction Gaëtan Le-Bel, Sergio Cortez Ghio, Danielle Larouche, Lucie Germain, and Sylvain L. Guérin 5. Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells Wenbo Wu and Ting Chen 6. Isolation and Characterization of Cutaneous Epithelial Stem Cells Stephanie R. Gillespie and David M. Owens 7. Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin Mariana T. Cerqueira, Ana M. Frias, Rui L. Reis, and Alexandra P. Marques 8. Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells Edwige Roy and Kiarash Khosrotehrani 9. Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis Stefano Sol, Dario Antonini, and Caterina Missero 10. Isolation and Cultivation of Epidermal (Stem) Cells Xusheng Wang, Shiyang Dong, and Yaojiong Wu 11. One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen Hua Qian, Xue Leng, Jie Wen, Qian Zhou, Xin Xu, and Xunwei Wu 12. Isolation and Cultivation of Skin-Derived Precursors Xiaoxiao Wang, Shiyang Dong, and Yaojiong Wu 13. Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells Petek Korkusuz, Sevil Köse, Nilgün Yersal, and Selin Önen 14. Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor Meilang Xue, Suat Dervish, and Christopher J. Jackson 15. Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair Mahmut Parmaksiz, Ayse Eser Elçin, and Yasar Murat Elçin 16. Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet Lei Chen, Daniel Radke, Shaohai Qi, and Feng Zhao 17. Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, and Amir Bajouri 18. Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, and Amir Bajouri 19. Skin Wound Healing: Refractory Wounds and Novel Solutions Gabriel M. Virador, Lola de Marcos, and Victoria M. Virador 20. Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice Lina Dagnino and Melissa Crawford 21. Melanoblasts as Multipotent Cells in Murine Skin Tsutomu Motohashi and Takahiro Kunisada 22. Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro Ke Yang, Weiming Qiu, Pei-Rong Gu, and Mingxing Lei 23. Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund, and Colin Jamora 24. Isolating Immune Cells from Mouse Embryonic Skin Ambika S. Kurbet and Srikala Raghavan 25. Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells Tsutomu Motohashi and Takahiro Kunisada 26. High-Titer Production of HIV-Based Lentivira sakriti opis