1. Introduction to FOXO Biology Wolfgang Link Part I: Biochemical and Molecular Methods 2. Characterization of microRNAs Regulating FOXO Expression Doug Hanniford and Eva Hernando 3. Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter Christopher P. Cardozo 4. Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover Thomas Heimbucher and Coleen T. Murphy 5. Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity Alba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, and Guillermo Velasco 6. Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes María Monsalve, Ignacio Prieto, Andreza Fabro de Bem, and Yolanda Olmos 7. Characterization of FOXO Acetylation Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, and Eric W-F Lam 8. Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays Antonia Ávila-Flores, Javier Arranz-Nicolás, and Isabel Mérida 9. Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes Vanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira 10. Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters Neeraj Kumar and Arnab Mukhopadhyay 11. RNAi Mediated Silencing of FoxO Factors Priyankar Sanphui, Akash Saha, and Subhas Chandra Biswas Part II: Imaging Approaches 12. Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO Francisco Castillo, Thomas A. Mackenzie, and Bastien Cautain 13. High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO Marta Barradas, Wolfgang Link, Diego Megías, and Pablo J. Fernández-Marcos 14. Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus Susana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link 15. Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology Anna C Blice-Baum, Georg Vogler, Meera C Viswanathan, Bosco Trinh, Worawan B Limpitikul, and Anthony Cammarato Part III: Omics and Bioinformatic Approaches 16. Genome-wide Analysis for Identifying FOXO Protein Binding Sites Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang 17. Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors Bradford E. Peercy and Martin F. Schneider 18. Following Transcriptome to Uncover FOXO Biological Functions Raymond Liang, Vijay Menon, and Saghi Ghaffari Part IV: Animal Models 19. Hydra as Model to Determine the Role of FoxO in Longevity Thomas C. G. Bosch 20. Genetic Ablation of FOXO in Mice to Investigate its Physiological Role Hwanhee Oh and Jihye Paik Part V: Human Gentic and Clinical Studies 21. Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity Timothy A Donlon, Philip MC Davy, and Bradley J Willcox 22. Analysis of FOXO3a Gene Polymorphism Associated with Asthma Tejas Shah and Kalpana Joshi sakriti opis