1. An Overview of Cholesterol Homeostasis Ingrid C. Gelissen and Andrew J. Brown 2. Hybrid In Silico/In Vitro Approaches for the Identification of Functional Cholesterol Binding Domains in Membrane Proteins Coralie Di Scala and Jacques Fantini 3. Structural Stringency of Cholesterol for Membrane Protein Function utilizing Stereoisomers as Novel Tools: A Review Md. Jafurulla and Amitabha Chattopadhyay 4. Manipulating Cholesterol Status within Cells Winnie Luu, Ingrid C. Gelissen, and Andrew J. Brown 5. Assaying Low-Density-Lipoprotein (LDL) Uptake into Cells Anke Loregger, Jessica Kristine Nelson, and Noam Zelcer 6. The use of L-sIDOL Transgenic Mice as a Murine Model to Study Hypercholesterolaemia and Atherosclerosis Eser J. Zerenturk and Anna C. Calkin 7. CRISPR/Cas9-mediated Generation of Niemann-Pick C1 Knockout Cell Line Ximing Du, Ivan Lukmantara, Hongyuan Yang 8. Quantitative Measurement of Cholesterol in Cell Population Using Flow Cytometry and Fluorescent Perfringolysin O Jian Li, Peter L. Lee, and Suzanne R. Pfeffe 9. Transport Assays for Sterol-binding Proteins: Stopped-flow Fluorescence Methods for Investigating Intracellular Cholesterol Transport Mechanisms of NPC2 Protein Leslie A McCauliff 1 and Judith Storch 10. Synthesis and Live-cell Imaging of Fluorescent Sterols for Analysis of Intracellular Cholesterol Transport Maciej Modzel, Frederik W. Lund, and Daniel Wüstner 11. Measurement of Cholesterol Transfer from Lysosome to Peroxisomes Using an In Vitro Reconstitution Assay Jie Luo, Ya-Cheng Liao, Jian Xiao, and Bao-Liang Song 12. Measurement of Mitochondrial Cholesterol Import using a Mitochondria-targeted CYP11A1 Fusion Construct Barry Edward Kennedy, Mark Charman, and Barbara Karten 13. Identifying Sterol Response Elements within Promoters of Genes Laura J. Sharpe and Andrew J. Brown 14. Membrane Extraction of HMG CoA Reductase as Determined by Susceptibility of Lumenal Epitope to In Vitro Protease Digestion Lindsey L. Morris and Russell A. DeBose-Boyd 15. Determining the Topology of Membrane-bound Proteins using PEGylation Vicky Howeand Andrew J Brown 16. Measuring Activity of Cholesterol Synthesis Enzymes Using Gas Chromatography/Mass Spectrometry Anika V. Prabhu, Winnie Luu, and Andrew J. Brown 17. Sterol Analysis by Quantitative Mass Spectrometry Andrew M. Jenner and Simon H.J. Brown 18. Measurement of Rates of Cholesterol and Fatty Acid Synthesis in vivo using Tritiated Water Adam M. Lopez, Jen-Chieh Chuang and Stephen D. Turley 19. Methods for Monitoring ABCA1-dependent Sterol Release Yoshio Yamauchi, Shinji Yokoyama, and Ta-Yuan Chang 20. ABC-transporter Mediated Sterol Export from Cells using Radio-Labeled Sterols Alryel Yang and Ingrid C. Gelissen 21. Measurement of Macrophage-specific In Vivo Reverse Cholesterol Transport in Mice M. Kockx, W. Jessup and L. Kritharides sakriti opis